Alexander Dane (Galaxy Quest) - as the composed, bullying spy who is haunted by a ghost.
Captain Jack Sparrow - as the unathletic, self-righteous assistant who fears a particular alien species.
Eric Delko (CSI: Miami) - as the nimble, frustrated manager.
Evil Gregor Mendel (Goats) - as the principled alien.
Inspector Stottlemeyer (Monk) - as the principled, humble repairman.
Jack Traven (Speed) - as the healthy, driven, whiny barbarian who is seeking meaning in life.
Lumiere (Beauty and the Beast) - as the tireless, creative, odd priest who suffers from a chronic medical condition.
McKay (SG Atlantis) - as the tireless laborer.
Mr. Big (Sex and the City) - as the unathletic miner who can't resist a fight.
Mystique (X-Men) - as the striking princess who is from a parallel dimension.
Nancy Blackett (Swallows and Amazons) - as the agile zookeeper whose species is being exterminated.
Neela (ER) - as the lazy herbologist who fears the future.
Rasputin - as the strong investor.
Sir Integra (Hellsing) - as the vicious prince haunted by flashbacks.
Wonder Woman - as the heroic coachman who was exiled to another dimension.